Inhibitory effect of phosphate on in vitro development of 2‐cell rat embryos is overcome by a factor(s) in oviductal extracts

FEBS Letters

Nishikimi, Akihiko¹  Yamada, Masayasu¹ 

[1] Laboratory of Reproductive Physiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan

关键词: Phosphate;    Oviductal extract;    Embryo development;    Hatching;    Rat;   

DOI  :  10.1016/S0014-5793(99)01510-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been found that inorganic phosphate (P) at concentrations as low as 10 μM markedly inhibits in vitro development of early rat embryos at the 1-cell or 2-cell stage to the blastocyst stage, although P is present at concentrations of 0.37–1.19 mM in oviductal fluid, in which the development of early embryos occurs. We show here that fractions (PTF, 50–250 μg/ml) of rat oviductal extracts (OVEs) passed through a Blue CL6B affinity column have the ability to overcome the inhibitory effects of P on the development of 2-cell rat embryos in a dose-dependent manner, whereas 250 μg/ml OVE or 250 μg/ml of the bound fractions (BF) induced degenerative changes in the embryos at the 2-cell stage. Moreover, PTF at concentrations of ≥100 μg/ml stimulated the hatching of blastocysts both in medium with and without P, although none of the blastocysts in medium without PTF hatched from their zona pellucida.

【 授权许可】

Unknown