Variation in hatching responses of Nematodirus battus eggs to temperature experiences

Parasites & Vectors

Sian Mitchell¹  Giles Innocent²  Lynsey A. Melville³  David J. Bartley³  Jan Van Dijk⁴ 

[1] Animal and Plant Health Agency, Job’s Well Road, Johnstown, SA31 3EZ, Carmarthen, UK;Biomathematics and Statistics Scotland, JCMB, King’s Buildings, Peter Guthrie Tait Road, EH9 3FD, Edinburgh, UK;Disease Control, Moredun Research Institute, Pentlands Science Park, Bush Loan, EH26 0PZ, Penicuik, UK;Zoetis, Birchwood Building, Springfield Drive, KT22 7LP, Leatherhead, UK;

关键词: Nematodirus battus;    Hatching;    Chill stimulus;    Climate change;    Seasonality;    Nematode;   

DOI  :  10.1186/s13071-020-04368-9

来源: Springer

【 摘 要 】

BackgroundNematodirus battus, unlike most other gastrointestinal nematodes, undergoes maturation to an infective larva within the egg. Historically, eggs were considered to require a period of chilling over winter followed by a period of temperature above 10 °C for synchronous hatching to occur (generally in spring). Anecdotal reports of Nematodirus infection out-with spring in veterinary journals and the farming press suggest that the concentrated pasture abundance of N. battus infective larvae may be changing. In order for control practices to be adapted, and unexpected disease outbreaks to be avoided, it is important to quantify how parasite epidemiology is changing and research the drivers behind it.MethodThe present study investigated the in vitro hatching response to temperature experiences (with and without a period of chilling) for egg samples of 90 N. battus populations obtained from 73 commercial sheep farms. Six aliquots of larvated eggs were prepared per population, three aliquots were placed at 4 °C for 6  weeks to provide a chill stimulus then incubated at the optimal hatching temperature for the species. The remaining three aliquots of eggs were incubated at the hatching temperature without a prior chill stimulus and the number of hatched larvae was compared between treatments.ResultsMedian hatch rate across all populations with chilling was 45% (95% CI: 42–48%) and without chilling was 4% (95% CI: 2–6%). Inter-population variation in hatching ranged from 0 to 87% of eggs able to hatch in the absence of a chill stimulus, mean non-chill hatching was 13 ± 2% of eggs (mean ± SE). Non-chill hatching rates were greater than chilled hatching rates in seven of the 90 populations tested.ConclusionsClearly, the variation in hatching responses to temperature experience is very large and therefore the seasonality of the parasite may vary not only between regions but also at farm level. In contrast to what previous work has suggested, there was a geographical trend towards higher non-chill hatching in the Northern parts of the UK.

【 授权许可】

CC BY