FEBS Letters | |
Two types of arrestins expressed in medaka rod photoreceptors | |
Tokunaga, Fumio1  Imanishi, Yoshikazu1  Hisatomi, Osamu1  | |
[1] Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cyo 1-1, Toyonaka, Osaka 560-0043, Japan | |
关键词: Arrestin; cDNA cloning; In situ hybridization; Immunocytochemistry; Medaka (Japanese killifish; Oryzias latipes); | |
DOI : 10.1016/S0014-5793(99)01483-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Similar to visual arrestins of other vertebrates, two subtypes of medaka visual arrestins, KfhArr-R1 and KfhArr-C, are selectively expressed in rods and cones, respectively [Hisatomi et al. (1997) FEBS Lett. 411, 12–18]. We isolated a cDNA encoding the third arrestin, KfhArr-R2, from a medaka retinal cDNA library. Phylogenetic analysis of arrestin sequences suggests that KfhArr-R2 is classified into the rod arrestin subtype. In situ hybridization indicated that KfhArr-R2 mRNA is localized in most of the rod photoreceptors, suggesting that both KfhArr-R1 and -R2 are co-expressed in rods. Antisera against KfhArr-R2 recognized outer segments, but anti-KfhArr-R1 antisera reacted with cell bodies and synaptic termini in light-adapted rods. It seems likely that KfhArr-R1 and -R2 play different roles in rod photoreceptors.
【 授权许可】
Unknown
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