Two types of arrestins expressed in medaka rod photoreceptors

FEBS Letters

Tokunaga, Fumio¹  Imanishi, Yoshikazu¹  Hisatomi, Osamu¹ 

[1] Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cyo 1-1, Toyonaka, Osaka 560-0043, Japan

关键词: Arrestin;    cDNA cloning;    In situ hybridization;    Immunocytochemistry;    Medaka (Japanese killifish;    Oryzias latipes);   

DOI  :  10.1016/S0014-5793(99)01483-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Similar to visual arrestins of other vertebrates, two subtypes of medaka visual arrestins, KfhArr-R1 and KfhArr-C, are selectively expressed in rods and cones, respectively [Hisatomi et al. (1997) FEBS Lett. 411, 12–18]. We isolated a cDNA encoding the third arrestin, KfhArr-R2, from a medaka retinal cDNA library. Phylogenetic analysis of arrestin sequences suggests that KfhArr-R2 is classified into the rod arrestin subtype. In situ hybridization indicated that KfhArr-R2 mRNA is localized in most of the rod photoreceptors, suggesting that both KfhArr-R1 and -R2 are co-expressed in rods. Antisera against KfhArr-R2 recognized outer segments, but anti-KfhArr-R1 antisera reacted with cell bodies and synaptic termini in light-adapted rods. It seems likely that KfhArr-R1 and -R2 play different roles in rod photoreceptors.

【 授权许可】

Unknown