Arrestins expressed in killifish photoreceptor cells

FEBS Letters

Satoh, Takunori¹  Tokunaga, Fumio¹  Imanishi, Yoshikazu¹  Hisatomi, Osamu¹ 

[1] Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cyo 1-1, Toyonaka, Osaka 560, Japan

关键词: Arrestin;    S-antigen;    cDNA cloning;    Killifish (or medaka;    Oryzias latipes);    In situ hybridization;   

DOI  :  10.1016/S0014-5793(97)00640-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two kinds of cDNA fragments (KfhArr-R and KfhArr-C) encoding the putative arrestins of killifish, Oryzias latipes, were isolated. The distributions of these transcripts were investigated by in situ hybridization, and it was demonstrated that KfhArr-R and KfhArr-C are expressed in, respectively, rod and all four types of cone cells. The deduced amino acid sequences of KfhArr-R and KfhArr-C are closely related to human S-antigen (rod arrestin) and X-arrestin (cone arrestin), respectively. Phylogenetic analysis of arrestin sequences suggests that vertebrate visual arrestins form a single cluster distinct from other arrestins and diverged to form rod and cone subtypes before the divergence between teleosts and tetrapods. It is speculated that the divergence pattern of vertebrate visual arrestins may prove to be reflected in the divergence of the proteins participating in the respective phototransduction cascades.

【 授权许可】

Unknown