Cloning, recombinant expression and biochemical characterization of the murine CD83 molecule which is specifically upregulated during dendritic cell maturation

FEBS Letters

Steinkasserer, Alexander²  Winklehner, Patrizia¹  Berchtold, Susanne²  Schuler, Gerold²  Heufler, Christine¹  Mühl-Zürbes, Petra² 

[1] Department of Dermatology, University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria;Department of Dermatology, University of Erlangen, Hartmannstrasse 14, 91052 Erlangen, Germany

关键词: CD83;    Dendritic cell;    cDNA;    Recombinant protein;    DC;    dendritic cells;    GM-CSF;    granulocyte-macrophage colony-stimulating factor;    LPS;    lipopolysaccharide;    PCR;    polymerase chain reaction;    TNF-α;    tumor necrosis factor α;    NMR;    nuclear magnetic resonance;   

DOI  :  10.1016/S0014-5793(99)01465-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human CD83 (hCD83) is a glycoprotein expressed predominantly on the surface of dendritic cells (DC) and represents the best marker for mature DC. Here, we report the cloning of the cDNA encoding mouse CD83 (mCD83) from a murine bone marrow-derived DC (BM-DC) cDNA library. DNA sequence analysis revealed a 196 amino acid protein including a signal peptide of 21 amino acids which shares 63% amino acid identity with hCD83. Using Northern blot analyses, mCD83 mRNA was found to be strongly expressed in mouse BM-DC and its expression was upregulated following stimulation with LPS or TNF-α. Transfection experiments using COS-7 cells revealed that mCD83 is glycosylated. Furthermore, the extracellular CD83 domain was recombinantly expressed in Escherichia coli and one-dimensional NMR data strongly support that the protein is structurally folded.

【 授权许可】

Unknown