Cloning and expression of the cDNA coding for the erythrocyte isoenzyme of human acylphosphatase

FEBS Letters

Raugei, Giovanni¹  Ramponi, Giampietro¹  Marzocchini, Riccardo¹  Cirri, Paolo¹  Fiaschi, Tania¹  Chiarugi, Paola¹ 

[1] Department of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134 Firenze, Italy

关键词: Acylphosphatase;    Recombinant protein;    cDNA;    Polymerase chain reaction;    PCR;    polymerase chain reaction;    bp;    base pair;    NMR;    nuclear magnetic resonance;    T3;    triiodothyronine;   

DOI  :  10.1016/0014-5793(95)00553-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three independent cDNAs coding for the erythrocyte isoform of human acylphosphatase were isolated and characterized. All the clones were incomplete at the 5′ end, but Northern blot analysis using the cDNA as a probe showed the presence of an unusually long mRNA 5′-untranslated region. The transcript was present in a variety of human cell lines of different origins, although at different levels. Southern blot analysis on DNA from different individuals revealed a simple hybridization pattern. Large amounts of pure enzyme with kinetic characteristics very similar to those of the native protein were expressed in E. coli.

【 授权许可】

Unknown