Spinach hexokinase I is located in the outer envelope membrane of plastids

FEBS Letters

Deppner, Heike²  Wiese, Anika¹  Hebbeker, Ulrike¹  Gröner, Ferdi¹  Sonnewald, Uwe²  Lerchl, Jens²  Flügge, Ulf-Ingo¹  Weber, Andreas¹ 

[1] Universität zu Köln, Lehrstuhl Botanik II, Gyrhofstr. 15, D-50931 Köln, Germany;Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466 Gatersleben, Germany

关键词: Hexokinase;    Plant;    Glucose transport;    Plastid envelope membrane;    Sugar signalling;    Subcellular localization;   

DOI  :  10.1016/S0014-5793(99)01417-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The subcellular localization of hexokinase activities in plant cells has been a matter of debate for a long time. We have isolated a hexokinase cDNA fragment from glucose-fed spinach leaves using a differential display reverse transcription-PCR approach. The corresponding cDNA was expressed in Escherichia coli and an antiserum, raised against the recombinant protein, was used in subcellular localization studies. The spinach hexokinase could be localized primarily to the outer envelope membrane of chloroplasts where it is inserted via its N-terminal membrane anchor. We suggest that the chloroplast envelope hexokinase is involved in the energization of glucose export from plastids rather than in the sugar-sensing pathway of the plant cell.

【 授权许可】

Unknown