Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium

FEBS Letters

Zhu, Ning¹  Olcese, Riccardo¹  Song, Min¹  Toro, Ligia¹  Stefani, Enrico¹  Barila, Bruna¹ 

[1] Department of Anesthesiology, UCLA School of Medicine, BH-509A CHS, Box 957115, Los Angeles, CA 90095-1778, USA

关键词: K channel;    Protein expression;    Myometrium;    Hormone;    mRNA;    MaxiK;    large conductance voltage-dependent and calcium-activated K+ channel;    hSlo;    human MaxiK;    PBS;    phosphate-buffered saline;    TBS;    Tris-buffered saline;    PAGE;    polyacrylamide gel electrophoresis;    IbTx;    iberiotoxin;   

DOI  :  10.1016/S0014-5793(99)01394-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Large conductance voltage-dependent and Ca²⁺-modulated K⁺ channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na⁺/K⁺-ATPase protein and RNA levels do not significantly change at different stages of pregnancy. The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition.

【 授权许可】

Unknown