Activation of Ras by phorbol esters in cardiac myocytes

FEBS Letters

Montessuit, Christophe¹  Thorburn, Andrew¹ 

[1] Department of Oncological Sciences, Huntsman Cancer Institute, 2000 E North Campus Drive, University of Utah, Salt Lake City, UT 84112-5550, USA

关键词: Cardiac myocyte;    Ras;    Protein kinase C;    Phorbol ester;    Myocardial hypertrophy;   

DOI  :  10.1016/S0014-5793(99)01223-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between protein kinase C (PKC) activation and Ras function was investigated in cardiac cells. Ras function was required for ERK activation by phorbol esters in cardiac myocytes, but not in cardiac fibroblasts. Accordingly, treatment with phorbol esters resulted in GTP loading of Ras in cardiac myocytes, but not fibroblasts. Ras activation by phorbol esters was abolished by a PKC specific inhibitor, but was insensitive to tyrosine kinase inhibitors. Ras activation was mediated by stimulation of guanine nucleotide exchange. These results suggest the existence of a novel pathway for Ras activation, specific to cardiac myocytes, with implications for myocardial hypertrophy.

【 授权许可】

Unknown