Cloning and characterization of two novel aldo‐keto reductases (AKR1C12 and AKR1C13) from mouse stomach

FEBS Letters

Okuda-Ashitaka, Emiko¹  Suzuki, Toshiko³  Watanabe, Kikuko³  Ikeda, Sakahiro⁴  Ito, Seiji¹  Shingu, Koh⁴  Masu, Yasuo¹  Nakao, Masafumi² 

[1] Department of Medical Chemistry, Kansai Medical University, Moriguchi 570-8506, Japan;Pharmacology Laboratories, Takeda Chemical Institute, Osaka 532-8686, Japan;Molecular Behavioral Biology, Osaka Bioscience Institute, Suita 565-0874, Japan;Department of Anesthesiology, Kansai Medical University, Moriguchi 570-8506, Japan

关键词: cDNA cloning;    Hydroxysteroid dehydrogenase;    Phylogeny;    Stomach;    Immunohistochemistry;    PG;    prostaglandin;    PGFS;    bovine lung PGF synthase;    HSD;    hydroxysteroid dehydrogenase;    CDR;    chlordecone reductase;    DD;    dihydrodiol dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)01243-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In contrast to hepatic hydrosteroid dehydrogenases (HSDs) of the aldo-keto reductase family (AKR1C), little is known about a stomach one. From a mouse stomach cDNA library, we isolated two clones encoding proteins of 323 amino acid residues. They exhibited 93.2% amino acid sequence identity and 64–68% with any known HSDs. Recombinant proteins expressed in Escherichia coli reduced 9,10-phenanthraquinone with NAD(P)H as cofactor. The mRNAs were exclusively expressed in stomach, liver and ileum. The present study demonstrates that these proteins are new members of the HSD subfamily and they are named AKR1C12 and AKR1C13. Immunohistochemical analysis suggests that they are involved in detoxification of xenobiotics in the stomach.

【 授权许可】

Unknown