Spliced leader‐associated RNA from Crithidia fasciculata contains a structure resembling stem/loop II of U1 snRNA

FEBS Letters

Schnare, Murray N.¹  Gray, Michael W.¹ 

[1] Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, N.S. B3H 4H7, Canada

关键词: Trypanosomatid;    Spliced leader-associated RNA;    Spliced leader;    Trans-splicing;    SL;    spliced leader;    sn;    small nuclear;    SLA;    spliced leader-associated;    RT;    reverse transcriptase;    DMSO;    dimethylsulfoxide;   

DOI  :  10.1016/S0014-5793(99)01235-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In contrast to earlier proposals, recent evidence suggests that trans-spliceosomes in trypanosomatid protozoa may contain a homolog of U1 small nuclear (sn) RNA (Schnare, M.N. and Gray, M.W. (1999) J. Biol. Chem. 274, 23 691–23 694). However, the candidate trypanosomatid U1 snRNA is unconventional because it lacks the highly conserved stem/loop II present in all other U1 snRNAs. Trypanosomatids also possess a unique spliced leader-associated (SLA) RNA of unknown function. We present the complete sequence of the SLA RNA from Crithidia fasciculata and propose that it may contribute a U1 snRNA-like stem/loop II to the trans-spliceosome.

【 授权许可】

Unknown