5′-Terminal chemical capping of spliced leader RNAs

TETRAHEDRON LETTERS

卷:53

Article

Piecyk, Karolina¹  Davis, Richard E.²  Jankowska-Anyszka, Marzena¹ 

[1] Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland

[2] Univ Colorado, Sch Med, Dept Biochem & Mol Genet, Aurora, CO 80045 USA

关键词: Cap analog;    5 '-Capped RNA;    TMG-capped RNA;    SL RNA;    Spliced leader;   

DOI  :  10.1016/j.tetlet.2012.06.127

来源: Elsevier

【 摘 要 】

Spliced leader (SL) RNA trans-splicing adds a N-2,N-2,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5' end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we present a chemical procedure for the preparation and purification of 5'-terminal capped MMG and TMG wild-type, and mutant 22 nt spliced leader RNAs (GGU/ACUUAAUUACCCAAGUUUGAG) with or without a 3' biotin tag. (C) 2012 Elsevier Ltd. All rights reserved.

【 授权许可】

Free