Specific loss of chondromodulin‐I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo

FEBS Letters

Shukunami, Chisa³  Tokunaga, Kunihiko¹  Takahashi, Hideaki E.¹  Endo, Naoto¹  Hiraki, Yuji³  Hayami, Tadashi¹  Kondo, Jun²  Mitsui, Kaori² 

[1] Department of Orthopaedic Surgery, Niigata University School of Medicine, Niigata 951-8510, Japan;Research Center, Mitsubishi Chemical Corporation, Kanagawa 227-0033, Japan;Department of Molecular Interaction and Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Chondromodulin-I;    Angiogenesis inhibitor;    Tumor angiogenesis;    Tumor suppression;   

DOI  :  10.1016/S0014-5793(99)01201-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chondromodulin-I (ChM-I) was previously identified as an angiogenesis inhibitor in cartilage. Here, we demonstrated that the level of ChM-I transcripts was substantially reduced to 100 or even less in the lower-grade chondrosarcomas, in articular cartilage or other benign cartilage tumors. We implanted human chondrosarcoma OUMS-27 cells into nude mice that reproducibly produced tumors with cartilaginous matrix. Tumor-induced angiogenesis was evident when the tumors were excised 30 days after implantation. However, the local administration of recombinant human ChM-I almost completely blocked vascular invasion and tumor growth in vivo. Moreover, ChM-I also inhibited the growth of HT-29 colon adenocarcinoma in vivo, implying its therapeutic potential for solid tumors.

【 授权许可】

Unknown