Generation of multiple transcripts from the chicken chondromodulin‐I gene and their expression during embryonic development

FEBS Letters

Shukunami, Chisa¹  Tanabe, Tadashi²  Hiraki, Yuji¹  Yamamoto, Saori² 

[1] Department of Molecular Interaction and Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan;Department of Pharmacology, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan

关键词: Chondromodulin-I;    Angiogenesis inhibitor;    Cartilage;    Morphogenese;   

DOI  :  10.1016/S0014-5793(99)00931-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chondromodulin-I (ChM-I) is an angiogenesis inhibitor isolated from fetal bovine cartilage. Here, we report the nucleotide sequence of chicken ChM-I cDNA. Chicken mature ChM-I had a significantly larger N-terminal hydrophilic domain than its mammalian counterparts. Chicken embryos expressed multiple transcripts (3.3, 2.0 and 1.7 kb in size) due to the alternative utilization of polyadenylation signals, whereas only the 1.7 kb transcripts were detected in mammals. Although confined to cartilage and eye at a later stage of development, whole-mount in situ hybridization revealed the expression of ChM-I mRNA in somites, heart, bronchial arches, roof plate, retina and limb buds. The expression pattern of the gene suggests a role for ChM-I in the morphogenesis during embryonic development.

【 授权许可】

Unknown