Stimulation of osteoblast proliferation by the cartilage‐derived growth promoting factors chondromodulin‐I and ‐II

FEBS Letters

Shukunami, Chisa³  Kakudo, Shinji²  Kumegawa, Masayoshi²  Kurokawa, Takahide¹  Mori, Yoshihisa²  Hiraki, Yuji³  Hakeda, Yoshiyuki²  Kagoshima, Masako²  Mano, Hiroshi²  Shiokawa, Miho²  Suzuki, Fujio³ 

[1] Department of Orthopaedic Surgery, Tokyo University Faculty of Medicine, Bunkyo, Tokyo 113, Japan;Department of Oral Anatomy, Meikai University School of Dentistry, Sakado, Saitama 350-02, Japan;Department of Biochemistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565, Japan

关键词: Chondromodulin-I;    Chondromodulin-II;    Osteoblast proliferation;    MC3T3-E1;    Alkaline phosphatase;   

DOI  :  10.1016/S0014-5793(97)00291-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously reported the isolation of the cartilage-derived growth promoting factors chondromodulin-I (ChM-I) and chondromodulin-II (ChM-II) from fetal bovine epiphyseal cartilage. Both of these factors stimulate the growth and matrix formation of chondrocytes in vitro. In the present study, we found that ChM-I and ChM-II stimulated the proliferation of clonal mouse osteoblastic MC3T3-E1 cells as well as primary mouse osteoblasts in culture. Unlike other known growth factors, these factors did not support the proliferation of fibroblasts. Concomitantly with growth stimulation of osteoblasts, there was a reduction of alkaline phosphatase (ALP) activity in the cells, the expression of the differentiated phenotype. These results suggest that epiphyseal cartilage may play a functional role in longitudinal bone growth by production of these unique growth-promoting factors.

【 授权许可】

Unknown