An RNA helicase (AtSUV3) is present in Arabidopsis thaliana mitochondria

FEBS Letters

Binder, Stefan²  Gagliardi, Dominique¹  Kuhn, Josef²  Spadinger, Ulrike²  Brennicke, Axel²  Leaver, Christopher J.¹ 

[1] Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK;Allgemeine Botanik, Universität Ulm, D-89069 Ulm, Germany

关键词: Plant mitochondrion;    RNA metabolism;    RNA helicase;    RNA processing;   

DOI  :  10.1016/S0014-5793(99)01168-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteins involved in mitochondrial mRNA processing and degradation in higher plants have yet to be identified. As a first step towards this aim, we report here the characterisation of a nuclear-encoded DExH box RNA helicase (AtSUV3) localised in Arabidopsis thaliana mitochondria. The AtSUV3 mRNA is assembled from the 16 exons of a weakly expressed unique gene and the predicted protein has a calculated molecular weight of 63.6 kDa. Subcellular fractionation of transgenic plants expressing AtSUV3/GUS fusion proteins localises this protein in mitochondria. The N-terminal domain of AtSUV3 containing the motifs characteristic of DExH box RNA helicases exhibits a low endogenous ATPase activity in vitro which can be stimulated by the presence of mitochondrial RNA, confirming that AtSUV3 is an RNA helicase.

【 授权许可】

Unknown