FEBS Letters | |
An RNA helicase (AtSUV3) is present in Arabidopsis thaliana mitochondria | |
Binder, Stefan2  Gagliardi, Dominique1  Kuhn, Josef2  Spadinger, Ulrike2  Brennicke, Axel2  Leaver, Christopher J.1  | |
[1] Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK;Allgemeine Botanik, Universität Ulm, D-89069 Ulm, Germany | |
关键词: Plant mitochondrion; RNA metabolism; RNA helicase; RNA processing; | |
DOI : 10.1016/S0014-5793(99)01168-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The proteins involved in mitochondrial mRNA processing and degradation in higher plants have yet to be identified. As a first step towards this aim, we report here the characterisation of a nuclear-encoded DExH box RNA helicase (AtSUV3) localised in Arabidopsis thaliana mitochondria. The AtSUV3 mRNA is assembled from the 16 exons of a weakly expressed unique gene and the predicted protein has a calculated molecular weight of 63.6 kDa. Subcellular fractionation of transgenic plants expressing AtSUV3/GUS fusion proteins localises this protein in mitochondria. The N-terminal domain of AtSUV3 containing the motifs characteristic of DExH box RNA helicases exhibits a low endogenous ATPase activity in vitro which can be stimulated by the presence of mitochondrial RNA, confirming that AtSUV3 is an RNA helicase.
【 授权许可】
Unknown
【 预 览 】
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RO201912020308324ZK.pdf | 513KB | download |