A mitochondrial dysfunction induces the expression of nuclear‐encoded complex I genes in engineered male sterile Arabidopsis thaliana

FEBS Letters

Gonzalez-Schain, Nahuel²  Busi, Maria V²  Gómez-Casati, Diego F²  Araya, Alejandro¹  Mouras, Armand³  Zabaleta, Eduardo J² 

[1] Laboratoire de Réplication et Expression des Génomes Eucaryotes et Rétroviraux (REGER), Centre National de la Recherche Scientifique and Université Victor Segalen Bordeaux 2, 146, rue Léo Saignat, Bât 3, 33076 Bordeaux, France;Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico de Chascomús (IIB-INTECH), Camino de Circunvalación Km 6, CC 164 (7130) Chascomús, Argentina;UMR 0619-Physiologie Biotechnologie Végétale, Institut National de la Recherche Agronomique et Université Victor Segalen Bordeaux 2, IBVM CR-INRA Bordeaux, B.P. 81, 33883 Villenave d'Ornon Cedex, France

关键词: Male sterility;    Plant mitochondrion;    Nuclear complex I gene;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(02)03631-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study the effect of a mitochondrial dysfunction induced by the expression of the unedited form of the subunit 9 of ATP synthase gene (u-atp9) in Arabidopsis, we constructed transgenic plants expressing u-atp9 under the control of three different promoters: CaMV 35S, apetala 3 and A9. The size and shape of transgenic plants bearing the apetala3::u-atp9 and A9::u-atp9 genes looked normal while the 35S::u-atp9 transformed plants showed a dwarf morphology. All u-atp9 expressing plants, independent of the promoter used, exhibited a male sterile phenotype. Molecular analysis of male sterile plants revealed the induction of the mitochondrial nuclear complex I (nCI) genes, psst, tyky and nadh binding protein (nadhbp), associated with a mitochondrial dysfunction. These results support the hypothesis that the expression of u-atp9 can induce male sterility and reveal that the apetala3::u-atp9 and A9::u-atp9 plants induced the sterile phenotype without affecting the vegetative development of Arabidopsis plants. Moreover, male sterile plants produced by this procedure are an interesting model to study the global changes generated by an engineered mitochondrial dysfunction at the transcriptome and proteome levels in Arabidopsis plants.

【 授权许可】

Unknown