Molecular characterization of Vig4/Vrg4 GDP‐mannose transporter of the yeast Saccharomyces cerevisiae

FEBS Letters

Hashimoto, Hitoshi¹  Yoda, Koji¹  Abe, Masato¹ 

[1] Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

关键词: Golgi membrane;    GDP-mannose transporter;    Yeast;    VRG4/VIG4;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(99)01177-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and vig4-2 had amino acid substitutions in the C-terminal region, Ala286Val and Ser278Cys, respectively. In accord with these mutations, trimming of the C-terminal hydrophobic part close to the region impaired the function and traffic of the proteins from the endoplasmic reticulum to the Golgi compartments.

【 授权许可】

Unknown