FEBS Letters | |
Molecular characterization of Vig4/Vrg4 GDP‐mannose transporter of the yeast Saccharomyces cerevisiae | |
Hashimoto, Hitoshi1  Yoda, Koji1  Abe, Masato1  | |
[1] Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan | |
关键词: Golgi membrane; GDP-mannose transporter; Yeast; VRG4/VIG4; Saccharomyces cerevisiae; | |
DOI : 10.1016/S0014-5793(99)01177-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and vig4-2 had amino acid substitutions in the C-terminal region, Ala286Val and Ser278Cys, respectively. In accord with these mutations, trimming of the C-terminal hydrophobic part close to the region impaired the function and traffic of the proteins from the endoplasmic reticulum to the Golgi compartments.
【 授权许可】
Unknown
【 预 览 】
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