Biosynthesis of ferulic acid esters of plant cell wall polysaccharides in endomembranes from parsley cells

FEBS Letters

Meyer, Knut¹  Kauss, Heinrich¹  Kohler, Annegret¹ 

[1] FB Biologie, Universität Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Germany

关键词: Parsley suspension cell;    Ferulic acid ester;    Cell wall polysaccharide;    Golgi membrane;    Feruloyl-CoA;    Fer;    ferulic acid;    Fer-CoA;    feruloyl-CoA;    IDPase;    inosine 5′-diphosphatase;    GSII;    1;    3-β-glucan synthase II;   

DOI  :  10.1016/0014-5793(91)81261-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A microsomal preparation from suspension-cultured parsley cells is able to transfer ferulic acid from the respective CoA thioester to endogenous acceptors. The reaction is not enhanced by digitonin but stimulated by Mg²⁺, Ca²⁺ and Co²⁺. Spermine can partly replace divalent ions. Solubility properties and degradation by polysaccharide hydrolases suggest that the products are polymeric cell wall carbohydrates. Sucrose density gradient centrifugation revealed that the most active vesicle fraction is distinct from plasma membranes but does also not peak with IDPase. It is suggested that a subfraction of the Golgi-apparatus is the source of enzyme and acceptors.

【 授权许可】

Unknown