The protein kinase inhibitor, K‐252a, decreases elicitor‐induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells

FEBS Letters

Conrath, Uwe¹  Kauss, Heinrich¹  Jeblick, Wolfgang¹ 

[1] FB Biologie, Universität Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Germany

关键词: Parsley suspension cell;    Signal transduction;    Elicitor-induced Ca2+ uptake;    K+ release;    Coumarin synthesis;    Protein kinase inhibitor;   

DOI  :  10.1016/0014-5793(91)80269-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An elicitor preparation from fungal cell walls known to induce coumarin synthesis in suspension-cultured parsley cells also elicits a rapid and transient Ca²⁺ uptake, K⁺ release and external alkalinization, and increases uptake of ⁴⁵Ca²⁺ into the cells. The latter three responses were inhibited by the protein kinase inhibitor K-252a at 0.2 μM. Elicitor-induced coumarin synthesis, a process which requires gene activation, was greatly enhanced by K-252a. These results suggest that protein phosphorylation might be involved in the initial steps of signal transduction as well as in the long-term induction of coumarin synthesis.

【 授权许可】

Unknown