FEBS Letters | |
The protein kinase inhibitor, K‐252a, decreases elicitor‐induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells | |
Conrath, Uwe1  Kauss, Heinrich1  Jeblick, Wolfgang1  | |
[1] FB Biologie, Universität Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Germany | |
关键词: Parsley suspension cell; Signal transduction; Elicitor-induced Ca2+ uptake; K+ release; Coumarin synthesis; Protein kinase inhibitor; | |
DOI : 10.1016/0014-5793(91)80269-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An elicitor preparation from fungal cell walls known to induce coumarin synthesis in suspension-cultured parsley cells also elicits a rapid and transient Ca2+ uptake, K+ release and external alkalinization, and increases uptake of 45Ca2+ into the cells. The latter three responses were inhibited by the protein kinase inhibitor K-252a at 0.2 μM. Elicitor-induced coumarin synthesis, a process which requires gene activation, was greatly enhanced by K-252a. These results suggest that protein phosphorylation might be involved in the initial steps of signal transduction as well as in the long-term induction of coumarin synthesis.
【 授权许可】
Unknown
【 预 览 】
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