cDNA cloning, characterization and stable expression of novel human brain carboxylesterase

FEBS Letters

Mori, Mieko¹  Chiba, Kan¹  Ogasawara, Yuko¹  Tsukada, Eiko¹  Hosokawa, Masakiyo¹ 

[1] Laboratory of Biochemical Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan

关键词: Carboxylesterase;    Human brain;    cDNA cloning;    Expression;    CES;    carboxylesterase;    D-MEM;    Dulbecco's modified Eagle's medium;    HPLC;    high-performance liquid chromatography;    5′-RACE;    5′-rapid amplification of cDNA end;   

DOI  :  10.1016/S0014-5793(99)01111-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNA sequence encoding a novel human brain carboxylesterase (CES) has been determined. The protein is predicted to have 567 amino acids, including conserved motifs, such as GEAGG, GXXXXFG, and GDGD which comprise a catalytic triad, and the endoplasmic reticulum retention motif (HXEL-COOH) observed in CES families. Their gene products exhibited hydrolase activity towards temocapril, p-nitrophenylacetate and long-chain acyl-CoA. Since the molecular masses of these gene products are similar to those that exist in capillary endothelial cells of the human brain [Yamamda et al. (1994) Brain Res. 658, 163–167], these CES isozymes may function as a blood-brain barrier to protect the central nervous system from ester or amide compounds.

【 授权许可】

Unknown