Molecular cloning, genomic characterization and expression of novel human α1A‐adrenoceptor isoforms

FEBS Letters

Khare, Reena¹  Clarke, David E¹  Shieh, Ing-Shih¹  Daniels, Donald V¹  Bhakta, Sunil¹  Jasper, Jeffrey R¹  Yamanishi, Susan S¹  Chang, Thomas K¹  Chan, Hardy W¹  Bach, Chinh¹  Lesnick, John D¹  Ford, Anthony P.D.W¹  Chang, David J¹  Kosaka, Alan H¹  Eglen, Richard M¹  Salazar, F.H.Rick¹ 

[1] Center for Biological Research, Neurobiology Unit, Roche Bioscience, 3401 Hillview Avenue, Palo Alto, CA 94304, USA

关键词: α1-Adrenoceptor;    Splice variant;    cDNA cloning;    Genome organization;    Expression;    PCR;    polymerase chain reaction;    CHO;    Chinese hamster ovary;    EDTA;    ethylene diamine tetraacetic acid;    RS-17503;    N-[2-(2-cyclopropylmethoxy-phenoxy)ethyl]-5-chloro-α;    α-dimethyl-1H-indole-3-ethanamine hydrochloride;    WB4101;    (2;    6-dimethoxyphenoxyethyl) aminomethyl-1;    4-benzodioxane hydrochloride;   

DOI  :  10.1016/S0014-5793(98)00024-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and characterized from human prostate novel splice variants of the human α_1A-adrenoceptor, several of which generate truncated products and one isoform, α_1A-4, which has the identical splice site as the three previously described isoforms. Long-PCR on human genomic DNA showed that the α_1A-4 exon is located between those encoding the α_1A-1 and α_1A-3 variants. CHO-K1 cells stably expressing α_1A-4 showed ligand binding properties similar to those of the other functional isoforms as well as agonist-stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that α_1A-4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

【 授权许可】

Unknown