Dissociation of the eukaryotic initiation factor‐4E/4E‐BP1 complex involves phosphorylation of 4E‐BP1 by an mTOR‐associated kinase

FEBS Letters

Denton, Richard M¹  Heesom, Kate J¹ 

[1] Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

关键词: mTOR;    4E-BP1;    Phosphorylation;    mRNA translation initiation;    Insulin;   

DOI  :  10.1016/S0014-5793(99)01094-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

mTOR immunoprecipitates contain two 4E-BP1 protein kinase activities. One appears to be due to mTOR itself and results in the phosphorylation of 4E-BP1 on residues T₃₆ and T₄₅, as shown previously by others. The other is a kinase which can be separated from mTOR and which phosphorylates 4E-BP1 within a peptide(s) containing residues S₆₄ and T₆₉. This phosphorylation, which occurs predominantly on S₆₄, results in the dissociation of 4E-BP1 from eIF-4E.

【 授权许可】

Unknown