A zinc‐finger transcription factor induced by TGF‐β promotes apoptotic cell death in epithelial Mv1Lu cells

FEBS Letters

Pons, Gabriel¹  Boxer, Linda M.²  Bartrons, Ramon¹  Chalaux, Elisabet¹  López-Rovira, Teresa¹  Ventura, Francesc¹  Rosa, Jose Luis¹ 

[1] Departament Ciències Fisiològiques II, Campus de Bellvitge, Universitat de Barcelona, C/ Feixa Llarga s/n., 08907 Hospitalet de Llobregat, Spain;Department of Medicine, University of Stanford, Stanford, CA 94305, USA

关键词: Transforming growth factor-β;    Apoptosis;    Transcriptional regulation;    TGF-β;    transforming growth factor-β;    BMP;    bone morphogenetic protein;    TIEG;    TGF-β induced transcription factor;    GIF;    GDNF induced factor;    PCR;    polymerase chain reaction;    GFP;    green fluorescent protein;   

DOI  :  10.1016/S0014-5793(99)01051-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transforming growth factor-β (TGF-β) superfamily members constitute a group of multifunctional factors that are able to stimulate apoptotic cell death in a variety of cells. In this report, we show that a zinc-finger transcription factor (TIEG) is an immediate early gene transcriptionally induced by TGF-β in the epithelial Mv1Lu cell line. We also demonstrate that, mimicking TGF-β effects, ectopic overexpression of TIEG is sufficient to trigger the apoptotic cell program in these cells, which is preceded by a decrease of Bcl-2 protein levels. Finally, apoptotic events elicited by TIEG overexpression can be effectively prevented by ectopic co-expression of Bcl-2. On the basis of these results we suggest that induction of TIEG expression has a role in the pro-apoptotic properties of TGF-β.

【 授权许可】

Unknown