cIAP‐1, but not XIAP, is cleaved by caspases during the apoptosis induced by TGF‐β in fetal rat hepatocytes

FEBS Letters

Herrera, Blanca¹  Fabregat, Isabel¹  Fernández, Margarita¹  Benito, Manuel¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Centro Mixto CSIC/UCM, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain

关键词: Inhibitor of apoptosis protein;    Hepatocyte;    Transforming growth factor-β;    Epidermal growth factor;    Apoptosis;    EGF;    epidermal growth factor;    IAP;    inhibitor of apoptosis proteins;    TGF-β;    transforming growth factor-β;   

DOI  :  10.1016/S0014-5793(02)02774-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the expression of XIAP, cIAP-1 and cIAP-2 in fetal rat hepatocytes and its possible regulation by pro-apoptotic stimuli (transforming growth factor-β (TGF-β)) and survival signals (epidermal growth factor (EGF)). The three forms of inhibitor of apoptosis proteins (IAPs) are expressed in fetal hepatocytes and only cIAP-1, but not XIAP or cIAP-2, is cleaved during TGF-β-induced apoptosis. The pan-caspase inhibitor Z-VAD.fmk blocked this effect, which indicates that cIAP-1 is a caspase substrate. EGF plays a dual role in the regulation of IAPs expression. On one hand, it increases cIAP-1 and cIAP-2 basal expression and, on the other hand, it blocks the cleavage of cIAP-1 by caspases induced by TGF-β.

【 授权许可】

Unknown