Proteinase 3 mRNA expression is induced in monocytes but not in neutrophils of patients with cystic fibrosis

FEBS Letters

Grimfeld, Alain²  Moog-Lutz, Christel¹  Houzel-Charavel, Anne¹  Cayre, Yvon E.¹  Just, Jocelyne¹  Witko-Sarsat, Véronique³  Canteloup, Sandrine³ 

[1] Inserm U 417, Hôpital Saint-Antoine, Bâtiment Raoul Kourilsky, 184 Rue du Faubourg St. Antoine, 75571 Paris Cedex 12, France;Service de Pneumologie et d'Allergologie Pédiatrique, Hôpital Trousseau, 26 Avenue du Dr Arnold Netter, 75571 Paris Cedex 12, France;Inserm U 507, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France

关键词: Proteinase 3;    Inflammation;    Lung disease;    Serine proteinase;    Myeloblastin;    Cystic fibrosis;    PR3;    proteinase 3;    CF;    cystic fibrosis;    NE;    neutrophil elastase;    SLPI;    secretory leucoprotease inhibitor;    MPO;    myeloperoxidase;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(99)01098-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte-derived PR3 in CF-associated airway inflammation.

【 授权许可】

Unknown