Low temperature and aging‐promoted expression of PUMP in potato tuber mitochondria

FEBS Letters

Maia, Ivan G.²  Catisti, Rosana¹  Fagian, Marcia M.¹  Vercesi, Anibal E.¹  Arruda, Paulo²  Nantes, Iseli L.¹ 

[1]Departamento de Patologia Clı́nica, NMCE, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil

[2]Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil

关键词: Plant uncoupling mitochondrial protein;    Thermogenesis;    Potato tuber mitochondrion;    Reactive oxygen;    ΔΨ;    mitochondrial transmembrane electrical potential;    ΔμH+;    proton electrochemical gradient;    BATM;    brown adipose tissue mitochondria;    BSA;    bovine serum albumin;    FA;    fatty acids;    LA;    linoleic acid;    PM;    potato mitochondria;    PUMP;    plant uncoupling mitochondrial protein;    UCP;    uncoupling proteins;   

DOI  :  10.1016/S0014-5793(99)01017-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28°C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4°C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential (ΔΨ). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on ΔΨ, the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.

【 授权许可】

Unknown