Induction of uncoupling protein (UCP) 2 in primary cultured hepatocytes

FEBS Letters

Saito, Masayuki¹  Kanehira, Katsushi¹  Kimura, Kazuhiro¹  Irie, Yukiko¹  Jung, Bae Dong¹  Cañas, Xavier¹ 

[1] Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan

关键词: Adenosine triphosphate;    Hepatocyte;    Mitochondrial respiration;    Uncoupler;    Uncoupling protein 2;    BAT;    brown adipose tissue;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    COX;    cytochrome c oxidase;    DNP;    dinitrophenol;    GAPDH;    glyceraldehyde 3-phosphate dehydrogenase;    FFA;    free fatty acid;    RCR;    respiratory control ratio;    UCP;    uncoupling protein;    WAT;    white adipose tissue;   

DOI  :  10.1016/S0014-5793(99)00987-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uncoupling protein 2 (UCP2) mRNA expression and function was examined in rat primary cultured hepatocytes. UCP2 mRNA was not expressed in freshly isolated hepatocytes, but appeared during a 24–144 h primary culture period. Isolated mitochondria from 144 h cultured hepatocytes showed a lower oxygen consumption rate in the presence of succinate and ADP. However, the ratio of the oxygen consumption rate when media contained succinate alone to that with succinate and ADP was increased by 166% versus control mitochondria. Moreover, the mitochondrial potential in the presence of succinate was decreased by 60%, indicating the potential role of UCP2 in hepatocyte mitochondria as an active uncoupler.

【 授权许可】

Unknown