Astrocyte differentiation mediated by LIF in cooperation with BMP2

FEBS Letters

Yanagisawa, Makoto¹  Arakawa, Hirokazu¹  Nakashima, Kinichi¹  Taga, Tetsuya¹ 

[1] Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan

关键词: gp130;    Leukemia inhibitory factor;    Bone morphogenetic protein;    Synergy;    Neuroepithelial cell;    Astrocyte;    IL-6;    interleukin-6;    LIF;    leukemia inhibitory factor;    STAT;    signal transducer and activator of transcription;    BMP;    bone morphogenetic protein;    GFAP;    glial fibrillary acidic protein;    RT-PCR;    reverse transcription and polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(99)00997-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. Here we show that astrocytes spontaneously emerge in vitro without exogenously added LIF and BMP2 in the culture of neuroepithelial cells for a much longer period. This spontaneous astrocyte differentiation is completely impaired when neuroepithelial cells deficient for gp130, a signal transducing receptor component for LIF, are used. We also show that LIF and BMP2 as well as related cytokines and respective receptor molecules are expressed in fetal mouse brain and cultured neuroepithelial cells. Taken together with our previous finding that prenatal mouse brain deficient for gp130 exhibits a severe reduction of astrocytes, it is suggested that LIF acts cooperatively with BMP2 in vivo to induce astrocyte differentiation in mouse developing brain.

【 授权许可】

Unknown