FEBS Letters | |
Inhibition of cell proliferation by lithium is associated with interference in cdc2 activation | |
Loomans, Dominique S.E1  Medema, René H1  Essers, Marieke A.G1  Klompmaker, Rob1  Rijksen, Gert1  Smits, Veronique A.J1  | |
[1] Jordan Laboratory, Department of Hematology, University Medical Center Utrecht G03.647, P.O. Box 85500, 3508 GA Utrecht, The Netherlands | |
关键词: Cell cycle; Checkpoint; Cyclin; G2; Lithium; DAPI; 4′; 6-diamidino-2-phenylindole; ECL; enhanced chemiluminescence; FACS; fluorescence activated cell sorter; GSK-3; glycogen synthase kinase 3; IMPase; inositol monophosphatase; PAA; polyacrylamide; RA; retinoic acid; | |
DOI : 10.1016/S0014-5793(99)01002-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Lithium can interfere with embryonal development in a variety of organisms. We investigated the effect of lithium on the proliferation of early embryonal cells. [3H]Thymidine incorporation of non-committed mouse P19 embryonal carcinoma cells was inhibited by lithium treatment. Similar effects were seen in a variety of other cells. This growth inhibition occurred in the G2 phase, since cells accumulated with a 4N DNA content, but the appearance of mitotic cells was blocked. Lithium could also prevent the activation of cdc2, thereby inhibiting cyclin B/cdc2 kinase activity. These data indicate that lithium might disturb embryonal development through interference in embryonal cell cycle regulation.
【 授权许可】
Unknown
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