Inhibition of cell proliferation by lithium is associated with interference in cdc2 activation

FEBS Letters

Loomans, Dominique S.E¹  Medema, René H¹  Essers, Marieke A.G¹  Klompmaker, Rob¹  Rijksen, Gert¹  Smits, Veronique A.J¹ 

[1] Jordan Laboratory, Department of Hematology, University Medical Center Utrecht G03.647, P.O. Box 85500, 3508 GA Utrecht, The Netherlands

关键词: Cell cycle;    Checkpoint;    Cyclin;    G2;    Lithium;    DAPI;    4′;    6-diamidino-2-phenylindole;    ECL;    enhanced chemiluminescence;    FACS;    fluorescence activated cell sorter;    GSK-3;    glycogen synthase kinase 3;    IMPase;    inositol monophosphatase;    PAA;    polyacrylamide;    RA;    retinoic acid;   

DOI  :  10.1016/S0014-5793(99)01002-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lithium can interfere with embryonal development in a variety of organisms. We investigated the effect of lithium on the proliferation of early embryonal cells. [³H]Thymidine incorporation of non-committed mouse P19 embryonal carcinoma cells was inhibited by lithium treatment. Similar effects were seen in a variety of other cells. This growth inhibition occurred in the G₂ phase, since cells accumulated with a 4N DNA content, but the appearance of mitotic cells was blocked. Lithium could also prevent the activation of cdc2, thereby inhibiting cyclin B/cdc2 kinase activity. These data indicate that lithium might disturb embryonal development through interference in embryonal cell cycle regulation.

【 授权许可】

Unknown