Cloning, tissue distribution, subcellular localization and overexpression of murine histidine‐rich Ca2+ binding protein

FEBS Letters

Ball, Judy K.¹  Ridgeway, Alan G.¹  Skerjanc, Ilona S.¹  Siu, Aaron¹  Petropoulos, Helen¹ 

[1] Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ont. N6A 5C1, Canada

关键词: Sarcoplasmic reticulum;    Ca2+ binding;    Leukemia;    Striated muscle;   

DOI  :  10.1016/S0014-5793(99)00993-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The histidine-rich Ca²⁺ binding protein (HRC) resides in the sarcoplasmic reticulum of muscle and binds Ca²⁺. Since Ca²⁺ concentrations can regulate gene expression via calcineurin, the mouse homologue of HRC (mHRC) was isolated and characterized. mHRC was detected in muscle progenitor cells, in primary clonal thymic tumors and a tumor cell line, suggesting a broader role for mHRC than in Ca²⁺ storage during muscle contraction. mHRC was present in the perinuclear region of myoblasts. To examine if it can regulate gene expression, mHRC was overexpressed in cells differentiating into cardiac and skeletal muscle. mHRC had no effect on cardiogenesis or myogenesis. Therefore, if mHRC plays a role in the regulation of gene expression during cellular differentiation, it does not appear to be either rate-limiting or inhibitory.

【 授权许可】

Unknown