Evidence that nebulin is a protein‐ruler in muscle thin filaments

FEBS Letters

Wardale, J.³  Leonard, K.²  Knight, P.³  Zeviani, M.¹  Labeit, S.²  Gibson, T.²  Trinick, J.³  Lakey, A.² 

[1] Instituto Neurologica C. Besta. V. Celoria II, I-20100, Milan, Italy;European Molecular Biology Laboratory, Meyerhofstr. 1.69 Heidelberg, Germany;Muscle and Collagen Research Group, Department of Veterinary Medicine, Bristol University, Langford, Bristol BS18 7DY, UK

关键词: Nebulin;    Striated muscle;    Thin filament;    Protein-ruler;   

DOI  :  10.1016/0014-5793(91)80503-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 × 35 = 245 residues. The repeat-motifs are likely to be largely α-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin from different species was found to vary in size in proportion to filament length. The data are consistent with the proposal that nebulin acts as a protein-ruler to regulate precise thin filament assembly.

【 授权许可】

Unknown