Serotonin 5‐HT1B and 5‐HT1D receptors form homodimers when expressed alone and heterodimers when co‐expressed

FEBS Letters

Xie, Zhidong¹  George, Susan R.¹  Lee, Samuel P.¹  O'Dowd, Brian F.¹ 

[1] Department of Pharmacology, University of Toronto, Room 4358, Medical Sciences Building, 1 King's College Circle, Toronto, Ont. M5S 1A8, Canada

关键词: G protein-coupled receptor;    Receptor dimerization;    Receptor oligomerization;    Western blotting;    Hetero-oligomerization;    5-HT;    5-hydroxytryptamine;    GPCR;    G protein-coupled receptor;    TM;    transmembrane;    PCR;    polymerase chain reaction;    5-CT;    5-carboxamidotryptamine;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    TBS;    Tris-buffered saline;    K i;    inhibitory binding constant;    GABA;    γ-aminobutyric acid;    GABABR;    metabotropic GABA receptor;   

DOI  :  10.1016/S0014-5793(99)00918-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The serotonin (5-hydroxytryptamine (5-HT)) 1B and 1D receptor subtypes share a high amino acid sequence identity and have similar ligand binding properties. In this study, we demonstrate that both receptor subtypes exist as monomers and homodimers when expressed alone and as monomers and heterodimers when co-expressed. Gene expression studies have shown that there are brain regions where the 5-HT_1B and 5-HT_1D receptors are co-localized and where heterodimerization may occur physiologically. This is the first direct visualization of the physical association between G protein-coupled receptors of different subtypes.

【 授权许可】

Unknown