Activation of gene transcription by tilapia prolactin variants tiPRL188 and tiPRL177

FEBS Letters

de Luze, Amaury²  Prunet, Patrick¹  Pezet, Alain²  Sandra, Olivier¹  Edery, Marc³  Sohm, Frédéric² 

[1] Laboratoire de Physiologie des Poissons, Institut National de la Recherche Agronomique, 35042 Rennes Cedex, France;Laboratoire de Physiologie Générale et Comparée, Muséum National d'Histoire Naturelle, Unité de Recherche Associée 90, Centre National de la Recherche Scientifique, 75231 Paris Cedex 05, France;Unité 344, Endocrinologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine Necker Enfants Malades, 156, rue de Vaugirard, 75730 Paris Cedex 15, France

关键词: Prolactin;    Signal transduction;    Receptor dimerization;    Fish;   

DOI  :  10.1016/S0014-5793(98)01285-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the tilapia species Oreochromis niloticus, the pituitary releases two forms of prolactins (tiPRL₁₈₈ and tiPRL₁₇₇). The binding parameters and the activation of tiPRL-induced JAK2/Stat5 signalling pathway were analysed using a mammalian cell line transiently transfected with the tiPRL receptor (tiPRLR). Our data indicate that the tiPRLR is able to mediate transcriptional activation of the PRL responsive element. At nanomolar concentrations, tiPRL₁₈₈ activates gene transcription whereas at micromolar concentrations it inhibits luciferase transcription from the lactogenic responsive element. This is consistent with a model of receptor dimerisation. In contrast, the activation by tiPRL₁₇₇ was only reached at high (μM) concentrations. The transcriptional activities induced by tiPRL₁₇₇ and tiPRL₁₈₈ are discussed in the context of the physiology of these hormones.

【 授权许可】

Unknown