Existence of uncoupling protein‐2 antigen in isolated mitochondria from various tissues

FEBS Letters

Řeháková, Zuzana³  Škobisová, Eva²  Žáčková, Markéta²  Tartaglia, Louis A.⁴  Gimeno, Ruth E.⁴  Garlid, Keith D.¹  Borecký, Jiří²  Brucknerová, Jana²  Růžička, Michal²  Ježek, Petr² 

[1] Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000, USA;Department of Membrane Transport Biophysics, Institute of Physiology, Vídeňská 1083, CZ 14220 Prague, Czech Republic;Department of Gnotobiology, Institute of Microbiology, Nový Hrádek, Czech Republic;Millennium Pharmaceuticals, 640 Memorial Drive, Cambridge, MA 02139, USA

关键词: Anti-UCP3 antibody;    Uncoupling protein 2;    Uncoupling protein 3;    BAT;    brown adipose tissue;    BCIP;    5-bromo-4-chloro-3-indolylphosphate;    DTT;    dithiothreitol;    EST;    expressed sequence tag;    IL-1β;    interleukin 1β;    IPTG;    isopropyl-β-d-thiogalactopyranoside;    MALDI-MS;    matrix-assisted laser dissorption/ionization mass spectroscopy;    NBT;    nitro blue tetrazolium;    SLS;    sodium lauroyl sarcosinate;    TNF;    tumor necrosis factor;    UCP;    uncoupling protein;    WAT;    white adipose tissue;   

DOI  :  10.1016/S0014-5793(99)00853-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs. The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated.

【 授权许可】

Unknown