Interaction with free β′ subunit unmasks DNA‐binding domain of RNA polymerase σ subunit

FEBS Letters

Goldfarb, Alex¹  Nikiforov, Vadim²  Kulbachinskiy, Andrey²  Mustaev, Arkady¹ 

[1]Public Health Research Institute, New York, NY 10016, USA

[2]Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia

关键词: RNA polymerase;    Promoter recognition;    UV cross-linking;    Escherichia coli;    RNAP;    RNA polymerase;    nt-oligo(s);    oligonucleotide(s) corresponding to the non-template strand of the −10 promoter region;    t-oligo(s);    oligonucleotide(s) corresponding to the template strand of the −10 promoter region;    ssDNA;    single-stranded DNA;   

DOI  :  10.1016/S0014-5793(99)00778-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The promoter recognition site on the σ⁷⁰ initiation factor is shielded from interaction with DNA unless σ⁷⁰ is bound to the core component of RNA polymerase (RNAP). It is shown that interaction of σ⁷⁰ with the isolated β′ subunit of Escherichia coli RNAP is sufficient to induce unshielding of the DNA binding site. Using UV-induced DNA-protein cross-linking we demonstrate that free β′ stimulates specific cross-links between region 2 of the σ⁷⁰ polypeptide and a fragment of the non-template promoter strand containing the TATAAT sequence. Thus the σβ′ subassembly of RNAP can assume a functionally competent conformation independently of the bulk of the RNAP core.

【 授权许可】

Unknown