Substitutions in the Escherichia coli RNA polymerase σ70 factor that affect recognition of extended −10 elements at promoters

FEBS Letters

Minchin, Stephen D¹  Sanderson, Andrew¹  Mitchell, Jennie E¹  Busby, Stephen J.W¹ 

[1] School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK

关键词: Bacterial transcription initiation;    RNA polymerase;    σ70 subunit;    Promoter recognition;    Extended −10 promoters;   

DOI  :  10.1016/S0014-5793(03)00500-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous work has shown that the base sequence of the DNA segment immediately upstream of the −10 hexamer at bacterial promoters (the extended −10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase σ⁷⁰ subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the σ⁷⁰ subunit can affect the recognition of different extended −10 element sequences.

【 授权许可】

Unknown