Molecular architecture of Manduca sexta midgut V1 ATPase visualized by electron microscopy

FEBS Letters

Radermacher, Michael²  Ruiz, Teresa²  Wieczorek, Helmut³  Grüber, Gerhard¹  Harvey, William R.¹ 

[1] Whitney Laboratory, University of Florida, St. Augustine, FL 32086, USA;Max-Planck-Institut für Biophysik, Abteilung Strukturbiologie, D-60528 Frankfurt am Main, Germany;Universität Osnabrück, Fachbereich Biologie/Chemie, D-49069 Osnabrück, Germany

关键词: V1 ATPase;    Electron microscopy;    Image processing;    Manduca sexta;    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(99)00739-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structure of the V₁ ATPase from the tobacco hornworm Manduca sexta has been determined from electron micrographs of isolated, negatively stained specimens. The resulting images clearly show a pseudohexagonal arrangement of six equal-sized protein densities, presumably representing the three copies each of subunits A and B, which comprise the headpiece of the enzyme. A seventh density could be observed either centrally or asymmetrically to the hexamer. The maximum diameter of the V₁ complex in the hexagonal projection is 13 nm with each of the six peripheral densities being 3–4 nm in diameter.

【 授权许可】

Unknown