Thin laminar microcrystals of 70 S ribosomes from Thermus thermophilus

FEBS Letters

Vasiliev, V.D.¹  Sergeev, Yu.V.¹  Shirokov, V.A.¹  Garber, M.B.¹  Ryazantsev, S.N.¹ 

[1] Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR

关键词: Ribosome microcrystal;    Electron microscopy;    Image processing;   

DOI  :  10.1016/0014-5793(89)81636-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new crystalline form, thin laminar microcrystals of 70 S ribosomes from Thermus thermophilus, have been obtained. Negatively stained ordered sheets derived from these crystals were studied by electron microscopy with subsequent computer image processing. The sheets have two-dimensional unit cell parameters a = b = 480 Å, γ = 90° and a resolution limit of about 30 Å. The microcrystals are suitable for three-dimensional image reconstruction.

【 授权许可】

Unknown