The jasmonate‐induced 60 kDa protein of barley exhibits N‐glycosidase activity in vivo

FEBS Letters

Wasternack, Claus¹  Parthier, Benno¹  Goerschen, Eckart¹  Dunaeva, Marina¹  Goebel, Cornelia¹ 

[1] Institut fur Pflanzenbiochemie, Weinberg 3, D-06120 Halle/Saale, Germany

关键词: Ribosome-inactivating protein;    RNA N-glycosidase;    Hordeum vulgare;    Nicotiana tabacum;    JA;    jasmonic acid;    JIP;    jasmonate-induced protein;    MJ;    methyl jasmonate;    RIP;    ribosome-inactivating protein;    eEF-2;    eukaryotic elongation factor 2;   

DOI  :  10.1016/S0014-5793(99)00645-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Upon jasmonate treatment barley leaf segments express a putative ribosome-inactivating protein (JIP60). The influence of this protein on translation in planta has been analysed by using barley plants and tobacco plants transformed with a barley cDNA encoding JIP60. In both plant systems JIP60 exhibited N-glycosidase activity in vivo. The depurination of the 25S rRNA of tobacco and barley ribosomes led to accumulation of translationally inactive polysomes.

【 授权许可】

Unknown