Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferase

FEBS Letters

Sprenger, Norbert¹  Schellenbaum, Luisa¹  van Dun, Kees²  Wiemken, Andres¹  Boller, Thomas¹ 

[1] Department of Botany, University of Basel, Hebelstrasse 1, CH‐4056 Basel, Switzerland;Department of Biotechnology, D.J. van der Have B.V., 4410 AA Rilland, The Netherlands

关键词: Fructan;    Sucrose:fructan 6-fructosyltransferase;    Nicotiana tabacum;    Cichorium intybus;    Hordeum vulgare;    1-FFT;    fructan:fructan 1-fructosyl transferase;    6-SFT;    sucrose:fructan 6-fructosyltransferase;    1-SST;    sucrose:sucrose 1-fructosyltransferase;    DP;    degree of polymerization;    DW;    dry weight;   

DOI  :  10.1016/S0014-5793(96)01418-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently cloned a cDNA encoding sucrose:fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the β-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652–11656]. Here we report functional expression of 6-SFT from barley in transgenic tobacco and chicory. Transformants of tobacco, a plant naturally unable to form fructans, synthesized the trisaccharide kestose and a series of unbranched fructans of the phlein type (β-2,6 linkages). Transformants of chicory, a plant naturally producing only unbranched fructans of the inulin type (β-2,1 linkages), synthesized in addition branched fructans of the graminan type, particularly the tetrasaccharide bifurcose which is also a main fructan in barley leaves.

【 授权许可】

Unknown