Mechanism and site of action of a ribosome‐inactivating protein type 1 from Dianthus barbatus which inactivates Escherichia coli ribosomes

FEBS Letters

Prestle, Jürgen¹  Hornung, Ellen¹  Mundry, Karl-Wolfgang¹  Schönfelder, Max¹ 

[1] Biological Institute, Department of Botany, Stuttgart University, Pfaffenwaldring 57, 7000 Stuttgart, 80, Germany

关键词: Escherichia coli 23 S rRNA;    Ribosome-inactivating protein;    RNA N-glycosidase;    Dianthin 29;   

DOI  :  10.1016/0014-5793(92)80549-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from 23 S rRNA. Nucleotide sequence studies showed that the site of N-glycosidic bond cleavage is at A-2660 within the universally conserved sequence 5′-AGUACGGAGGA-3′ near the 3′-end of 23/28 S rRNAs. To our knowledge, Dianthin 29 is the first ribosome-inactivating protein which is shown to inactivate intact prokaryotic ribosomes in the same manner as eukaryotic ribosomes.

【 授权许可】

Unknown