Induction of cytokines in a human colon epithelial cell line by Shiga toxin 1 (Stx1) and Stx2 but not by non‐toxic mutant Stx1 which lacks N‐glycosidase activity

FEBS Letters

Natori, Yumiko¹  Ohmura, Mari¹  Zeng, Xun-Ting¹  Yamasaki, Chisato¹  Yamasaki, Shinji¹  Takeda, Yoshifumi¹  Natori, Yasuhiro¹ 

[1]Department of Clinical Pharmacology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan

关键词: Shiga toxin;    Cytokine;    Interleukin-8;    RNA N-glycosidase;    Colon epithelial cell;    Shiga toxin-producing Escherichia coli;    CD50;    50% cytotoxic dose;    Gb3;    globotriaosylceramide;    IL-8;    interleukin-8;    LPS;    lipopolysaccharide;    MCP-1;    monocyte chemoattractant protein-1;    MIP-1α;    macrophage inflammatory protein-1α;    RT-PCR;    reverse transcription polymerase chain reaction;    Stx;    Shiga toxin;    STEC;    Shiga toxin-producing Escherichia coli;    TNF-α;    tumor necrosis factor-α;   

DOI  :  10.1016/S0014-5793(98)01667-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stx1 and Stx2 produced by Shiga toxin-producing Escherichia coli are cytotoxic due to their N-glycosidase activity on 28S rRNA. In this study, we have shown that proinflammatory cytokine mRNAs, especially IL-8, were induced by Stx1 and Stx2 in Caco-2 cells. A non-toxic mutant of Stx1 which lacks N-glycosidase activity did not induce cytokine mRNAs. IL-8 production at the protein level was enhanced by Stx1 and Stx2, but not by the mutant Stx1. These results demonstrate that Shiga toxins induce expression and synthesis of cytokines in Caco-2 cells and their N-glycosidase activity is essential for the induction.

【 授权许可】

Unknown