Influenza virus A infection induces interleukin‐8 gene expression in human airway epitheial cells

FEBS Letters

Jacoby, David B.¹  Choi, Augustine M.K.¹ 

[1] Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD, USA

关键词: Interleukin-8;    Airway epithelial cell;    Virus;    Gene expression;    Inflammation;    Cytokine;   

DOI  :  10.1016/0014-5793(92)80799-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemstactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-folsd. Thus induction or interleukin-8 gene expression in virus-infected airway epithelium may be an important early step lending to virus-induced airway inflammation.

【 授权许可】

Unknown