Calcium ionophore A23187 induces interleukin‐8 gene expression and protein secretion in human monocytic cells

FEBS Letters

Kellie, Stuart¹  Butcher, Christopher J.¹  Wilson, Lynn¹ 

[1] Yamanouchi Research Institute, Littlemore Hospital, Oxford, 0X4 4XN, UK

关键词: Interleukin-8;    Gene expression;    Monocyte;   

DOI  :  10.1016/0014-5793(93)81092-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The regulation of the expression of the interleukin-8 (IL-8) gene in human monocytic cell lines has been investigated. Agents such as interleukin-1 (IL-1) or interferon-γ (IFNγ) did not induce increased IL-8 expression in THP-1 or U937 cells. Bacterial lipopolysaccharide endotoxin (LPS) or phorbol myristate acetate (PMA) alone induced suboptimal expression as assessed by Northern blotting; however, preincubation of cells with PMA followed by endotoxin induced much higher levels of IL-8 mRNA. Incubation of the cells with the calcium ionophore A23187 resulted in consistent increased IL-8 gene expression comparable to that of cells treated with endotoxin alone. In addition to inducing IL-8 mRNA this calcium ionophore also induced IL-8 protein synthesis as assessed by immunofluorescence and secretion as detected by ELISA. These results indicate that increases in intracellular calcium result in IL-8 gene expression and protein secretion.

【 授权许可】

Unknown