Chromatin structural transitions in Drosophila embryo cell‐free extract result in a high conformational flexibility of nucleosomal DNA

FEBS Letters

Krajewski, Wladyslaw A¹ 

[1] Institute of Developmental Biology, Russian Academy of Sciences, Vavilova Str. 26, 117808 Moscow, Russia

关键词: Chromatin;    Nucleosome;    DNA topology;    Nucleosome remodelling;   

DOI  :  10.1016/S0014-5793(99)00637-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA within chromatin has considerably more restricted flexibility in comparison with naked DNA. This raises the main question of how the functioning multi-enzyme complexes overcome the nucleosomal level of DNA packaging. We studied the DNA conformational flexibility of reconstituted chromatin in a cell-free system derived from Drosophila embryo extracts. Using this system, we have found evidence for a energy- independent chromatin remodelling process that efficiently destabilizes the nucleosome structure resulting in a high conformational flexibility of nucleosomal DNA. The described chromatin remodelling process may lay on the basis of defined molecular principles governing the molecular heterogeneity of chromatin structures in vivo.

【 授权许可】

Unknown