Human stromelysin gene promoter activity is modulated by transcription factor ZBP‐89

FEBS Letters

Henney, Adriano¹  Whatling, Carl¹  Watkins, Hugh¹  Ye, Shu¹ 

[1] Department of Cardiovascular Medicine, University of Oxford, Windmill Road, Oxford OX3 7BN, UK

关键词: Matrix metalloproteinase;    Gene regulation;    Transcription factor;    Tumor invasion;    Metastasis;    ZBP-89;    89-kDa zinc binding protein;    MMP;    matrix metalloproteinase;    TIMP;    tissue inhibitor of metalloproteinases;    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    NGF;    nerve growth factor;    IL-1;    interleukin-1;    AP-1;    activator protein-1;    PEA3;    polyomavirus enhancer A binding protein-3;    SPRE;    stromelysin PDGF-responsive element;    3′-RACE;    rapid amplification of 3′-cDNA end;    EMSA;    electrophoretic mobility shift assay;    PMSF;    phenylmethylsulphonyl fluoride;   

DOI  :  10.1016/S0014-5793(99)00509-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Matrix metalloproteinase expression is under strict regulation in physiological conditions. Disruption of the regulatory mechanisms can lead to tissue destruction and is associated with tumour invasion and metastasis. Using the one-hybrid assay technique with a cis-element in the promoter region of the stromelysin (matrix metalloproteinase-3) gene, a cDNA encoding a transcription factor termed ZBP-89 was obtained. The interaction between ZBP-89 and the stromelysin promoter element was confirmed by electrophoretic mobility shift assays with a recombinant ZBP-89. Reporter gene expression under the control of the stromelysin promoter in transiently transfected cells was significantly increased when the cells were co-transfected with a ZBP-89 expression construct. These results indicate that ZBP-89 interacts with the stromelysin promoter and upregulates its activity. As ZBP-89 expression is known to be increased in gastric carcinoma cells, induction of stromelysin expression may be a significant factor in tumour metastasis.

【 授权许可】

Unknown