Real‐time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein

FEBS Letters

Nakanishi, Tomoko²  Nishimune, Yoshitake¹  Okabe, Masaru²  Yamada, Shuichi⁵  Parvinen, Martti³  Ikawa, Masahito²  Baba, Tadashi⁴ 

[1] Research Institute for Microbial Diseases, Osaka University, Osaka, Japan;Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan;Department of Anatomy, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland;Institute of Applied Biochemistry and Tsukuba Advanced Research Alliance, University of Tsukuba, and the National Institute for Advanced Interdisciplinary Research, Tsukuba Science City, Ibaraki 305-8572, Japan;Program for Promotion of Basic Research Activities for Innovative Biosciences, Tokyo, Japan

关键词: Sperm;    Acrosome reaction;    Fertilization;    Green fluorescent protein;   

DOI  :  10.1016/S0014-5793(99)00433-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual ‘live’ sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.

【 授权许可】

Unknown