Clusterin (SGP‐2) gene expression is cell cycle dependent in normal human dermal fibroblasts

FEBS Letters

Bettuzzi, S¹  Tiozzo, R¹  Moretti, M¹  Astancolle, S¹  Guidetti, G¹  Corti, A¹ 

[1] Dipartimento di Scienze Biomediche, University of Modena and Reggio Emilia, Via G. Campi 287, 41100 Modena, Italy

关键词: SGP-2;    Clusterin;    Human dermal fibroblast;    Cell cycle;    Human growth arrest specific protein 1;    Histone H3;    SGP-2;    sulfated glycoprotein 2 (clusterin);    FCS;    fetal calf serum;    DMEM;    Dulbecco's modified Eagle's medium;    BrdU;    5′-bromo-2′-deoxyuridine;    PI;    propidium iodide;    h-gas1;    human growth arrest specific protein 1;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    PHA;    phytohemoagglutinin;    ODC;    ornithine decarboxylase;   

DOI  :  10.1016/S0014-5793(99)00375-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In confluent human dermal fibroblasts brought to quiescence (G₀) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G₂/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G₀ phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G₀, in parallel with h-gas1 mRNA. At 6 h (roughly G₁ phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G₀ peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.

【 授权许可】

Unknown