A novel inhibitor protein for Bombyx cysteine proteinase is homologous to propeptide regions of cysteine proteinases

FEBS Letters

Kageyama, Takashi²  Yamamoto, Yoshimi¹  Takahashi, Susumu Y¹  Watabe, Shoji³ 

[1] Department of Biochemistry and Radiation Biology, College of Agriculture, Yamaguchi University, Yamaguchi 753, Japan;Department of Biochemistry, Primate Research Institute, Kyoto University, Inuyama, Aichi 484, Japan;Radioisotopes Laboratory, Yamaguchi University, Yamaguchi 753, Japan

关键词: Inhibitor;    Cysteine proteinase;    Propeptide region;    cDNA cloning;    Sequence homology;   

DOI  :  10.1016/S0014-5793(99)00382-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone for an inhibitor of Bombyx cysteine proteinase was isolated and sequenced. Active inhibitor proteins were expressed in Escherichia coli using the cDNA. The open reading frame of the cDNA encodes a 105 residues protein with 19 residues of a signal sequence. The inhibitor has amino acid sequences homologous to several cysteine proteinases, but only to their propeptide sequences. The results suggest that some cysteine proteinase proregions may have evolved as autonomous modules and become inhibitor proteins for cysteine proteinases.

【 授权许可】

Unknown